地高辛高效DNA標記及檢測試劑盒II型

產品編號 11585614910 | ROCHE

DIG-High Prime DNA Labeling and Detection Starter Kit II 

產品簡介：

貨號：11585614910

產品品牌：roche

產品名稱：地高辛高效DNA標記及檢測試劑盒II型（化學發光法）

上?，F貨市場特價供應

產品明細：

詳細說明：

辛高素標記的探針，可用于在南方，北方和點雜交分析，以及殖民地和菌斑雜交。該套件提供了與DIG-11-dUTP標記的隨機引物標記的DNA模板，堿不穩定，顏色DIG標記的雜交檢測。本試劑盒組裝方便考慮，提供一個現成的封閉液，NBT / BCIP聯合原液，挖易HYB顆粒。挖總理混合物，包括穩定Klenow酶，核苷酸，引物，反應緩沖液，在一個方便的試劑。

靈敏度和特異性：該標準的檢測采用線性pBR328 1微克。這是通常的期望打成0.8微克DNA標簽后1小時，2.3微克的DNA標記后20小時的反應。0.1同源的DNA PG在斑點雜交，檢測到后16小時的色彩發展（探針濃度為20毫微克/毫升）。一個單拷貝人類基因（TPA）是在Southern雜交檢測，裝載1微克消化胎盤DNA

內容

1.  高辛，總理，濃度5倍，50μL

2.  DIG標記的對照DNA，20μL（5微克/毫升），pBR328（與線性巴姆您好）

3.  DNA稀釋液，3×1毫升

4.  抗地高辛-AP的共軛，100μL

5.  NBT / BCIP聯合解決方案，濃，6×1毫升

6.  封閉液，10倍濃度，4×100毫升。

7.  挖易的HYB沖劑，4×100毫升

電  話：      

傳  真：
郵  編：200233
地  址：上海市欽江路15號14層
郵  箱：solarbio
網  址：www.5ca006.com

產品名稱

Application

DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled probes are generated at high yield within one hour or after overnight incubation. DIG-High Prime labeled DNA probes are used in a variety of hybridization techniques: • Southern blots
• Northern blots
• Dot blots
• Colony and plaque hybridizations
• For all types of filter hybridization
• For single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat

General description

下載特定產品批次和規格的CofA、SDS和IFU。

Convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

僅用于生命科學研究。不可用于診斷。

包裝

1 kit containing 7 components.

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.

性質

 試劑 質量放心 價格低 為中國生物產業做貢獻  

上海索寶特價 【】